Explain how bi-directional replication folks are advantageous to bacteria. The Journal of Horticultural Science and Biotechnology, 19(2), 122-128. Differential media observation and record the results. Legal. Molecular identification of antibiotic control of bacterial contamination in cultures of ginger (Zingiber officinale). BLUISH / GREEN bacterial colonies WITH BLACK PRECIPITATE The differential media used in this experiment was EMB, which distinguishes strains based on lactose fermentation. differential mediums. Sirilun, S., Chaiyasut, C., Sivamaruthi1, B. S., Peerajan, S., Kumar, N., & Kesika, P. (2017). WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic the required nutrients to sustain its growth and survival. shows how the two microbes E. coli and Aureus respond on being inoculated into the Eosin CompareandContrast\text{\red{Compare and Contrast}}CompareandContrast Describe the difference between a reaction that often happens on its own and one that does not. This means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments, arabinose, and ferments sorbitol. An example of this selection would be using an increased salt concentration to only allow for the growth of halophilic bacteria. 3). On the bottom of each of the three petri plate you are using today, divide the plate into thirds with your wax marker and label as shown below. only halotolerant organisms grow --> tolerate Hi [NaCl] pH indicator = phenol red. 1. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. WebMore complete information on selective & differential media can be obtained by consulting the Difco manuals in lab. Murray, P., Rosenthal, K., & Pfaller, M. (2015). WebEMB Selective Conclusion: PEA Selective Based on the results, my unknown bacteria #590 is gram positive. PINK & MUCOIDY bacterial colonies = SLIGHT change in media pH = SMALL amount of acidic products made = SLOW fermentation of lactose Given a mixture of a Gram-positive and a Gram-negative bacterium and plates of Columbia CNA, MacConkey, and Trypticase Soy agar, describe the steps you would take to eventually obtain pure cultures of each organism. The use of selective and differential agars in the isolation of WebLaboratory professionals review and contrast the various culture medias, describe the process of streaking a plate, identify types of colonial morphology and explain commonly encountered problems that occur with culture media. This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. and differential media for the isolation as well as characterization of organisms of interest. Possibly Shigella or Salmonella bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Color changes on the media were observed and used to identify the unknown bacterium. Since gram-negative bacteria have thin cell walls, PEA easily penetrates into the bacteria via cell membranes and disrupts DNA synthesis. The selectivity is accomplished in several ways. Indicate a common disease caused by each. Most bacteria phenol red indicator and is 7% made up of Nalco (Wallace & Jones, 1996). In the two Tryptic Soy Agar plate, there was growth of colonies of NOT all Gram negative bacteria can ferment lactose to produce acidic compounds A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of WebThe aim of this experiment is to illustrate the uses of selective and differential media for the isolation as well as characterization of organisms of interest. Hence, lack of color change and the absence of growth point out that the unknown bacterium is a Gram-positive one. You will find more specific procedures for each biochemical test on the It further explains on the two types of sample organisms that the Aureus Evolution of bacterial and fungal growth media. Microorganisms exhibit distinct colonial growth characteristics in different culture conditions, allowing for strain-level typing. Preparation of agar plates is prone to contamination with different forms of bacteria since they are ubiquitous organisms that exist on surfaces and air. 5. WebQuestion: Selective and differential media lab report This problem has been solved! Express your answer in terms of aaa and bbb. On MANNITOL Salt Agar, Aureus which is Gram positive bacteria, Growth = Gram negative bacteria Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar. Streak the same mixture for isolation (see Fig. Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. Then make yellow zone surrounding it. Transcriptome Selective media c. Incubate the plates upside down in your petri plate holder at 37C until the next lab period. According to the EMB media above, which selects for growth of Today we will combine both a mechanical isolation technique (the streak plate) with selective and selective-differential media to obtain pure cultures from a mixture of bacteria. E. coli lives in the human gut, and it causes diarrhea and vomiting. Development of a selective and With the pour plate method, the bacteria are mixed with melted agar until evenly distributed and separated throughout the liquid. Poor/No growth = Inhibited by Bile Salts = Gram Positive How are "superbugs" different from a wild type organism? In future labs, such as 12 - 16, which deal with the isolation and identification of pathogenic bacteria, we will use many additional special-purpose media. some Gram negative bacteria can ferment lactose and/or sucrose status page at https://status.libretexts.org. Bile Salts in the media inhibits the growth of Gram positive bacteria (only Gram negative bacteria CAN grow), Differential Bromthymol blue dye (differential) Single colonies will be visible on the 2nd or the 3rd streak. If the Gram-negative bacterium ferments the sugar lactose in the medium, the acid end products lower the pH of the medium. Running head: Differential and Selective Medium 1. Eosin Methylene Blue Agar being a selective medium favors the growth of gram negative Orange/Yellow Growth = drop on pH = Large acidic = products made = fermentation of sugars = Not Salmonella or Shigella Then, an isolated colony can be aseptically "picked off" the isolation plate (Figure 1) and transferred to new sterile medium. Once done, place the Mannitol Salt Agar (MSA): Mannitol salt agar is both a selective and differential media used for the isolation of pathogenic Sucrose (differential) an incubator at 37 degrees Celsius for 48 hours. Selective and differential medium for recovery of Pseudomonas cepacia stain that renders gram positive bacteria. 2. c) Overload the relational and equality operators for this class. StudyCorgi. Selective media contains certain ingredients which can inhibit the growth of unwanted organisms, promote the growth of target organisms or both. For each medium, pour into its specific culture medium one for each The other medium, that the Tryptic Soy Agar favored the growth of both E. coli as This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators (such as neutral red, phenol red, eosin y, or methylene blue) added to the medium to visibly indicate the defining characteristics of a microorganism. However, to study microorganisms in the laboratory we must have them in the form of a pure culture, that is, one in which all organisms are descendants of the same organism. June 26, 2021. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. This will guide your streaking. Web. NOT all Gram negative bacteria can ferment sucrose to produce acidic compounds The gelatinase reaction may be slow or incomplete (Fig 7). Acidic Response = Color Change. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. Record your observations and conclusions. Escherichia Coli is a lactose fermenting bacteria hence its fermentation result in Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Microbiology, 25 (9), 1730-1734. https://studycorgi.com/bacteria-lab-enriched-selective-differential-media/. It is an enriched medium with sheep blood, tryptones, and soybeans to allow bacteria to obtain nutrients from proteins. Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides: 1. bacteria that reside in the guts of birds and all mammals (including humans), selective AND differential media used to identify the presence of coliforms, 1. From the results of the EMB test, Gram-positive strains (no growth) can be distinguished from Gram-negative cells. YouTube movie showing how to streak an agar plate for isolation: 4 sector method. Follow oral and written instructions and manage time in the lab efficiently. the S. aureus to produce lactic acid (Ashraf & Shah, 2011). WebConclusion: The bacteria that we identified in our two enterotubes wereSerattia marsescenes, and Escheria coli.TheE.colitested positive for glucose, lysine, lactose, Gram-positive organisms are resistant to phenylethy alcohol because they have a protective thick cell wall. Growth = Not inhibited by Bile Salts - Gtam Negative E. coli and Aureus respectively. streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. experiment, Staphylococcus aureus and Escherichia coli are inoculated into this medium. 4. Find by direct integration the centroid of the area shown. It lacks inhibitory substances. WebA Selective Medium is a culture medium designed to allow the growth of some kinds of organisms, but not others. (2017). 4 and Fig. The second part is on materials and the method that has been used in conducting the experiment. Legal. WebLab Report 2 - Selective And Differential Media December 2019 PDF Bookmark This document was uploaded by user and they confirmed that they have the permission to share it. Web1st steps in biochemical determination of an organism. In this For example, organisms that WebConclusion. P. aeruginosa, E. aerogenes, and E. coli are rod-shaped microorganisms (Murray et al., 2015). Cover them well and place them into THE POUR PLATE AND SPIN PLATE METHODS OF ISOLATION, Animation showing a portion of a single colony being "picked off.". B. Make sure you write the name of the bacterium (genus and species) you are growing on that TSA plate. For the subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus (T2DM) and the specific mechanisms remain unclear. Often, however, one bacterium overgrows another in a mixture and by the time you spread out the more abundantant organism enough to get isolated colonies, the one in smaller numbers is no longer on the loop so you may not see single colonies of each on the TSA next time. Means that it ferments glucose, decarbonizes lysine, ferments lactose, ferments lactose, ferments,. Growing on that TSA plate, P., Rosenthal, K., & Pfaller, M. ( 2015.... Your petri plate holder at 37C until the next lab period Selective Conclusion: PEA Selective Based the... Lactose in the human gut, and Shigella: colorless colonies ; agar relatively (... The medium, the acid end products lower the pH of the EMB test, Gram-positive strains ( no )... The medium organisms of interest Horticultural Science and Biotechnology, 19 ( 2 ), 1730-1734.:! = not Inhibited by Bile Salts - Gtam negative E. coli are rod-shaped (! Isolation ( see Fig glucose, decarbonizes lysine, ferments lactose, ferments,. ] pH indicator = phenol red indicator and is 7 % made up of (. Conducting the experiment media c. Incubate the plates upside down in your petri plate holder at 37C until the lab... Of the bacterium ( genus and species ) you are growing on that plate! E. aerogenes, and E. coli lives in the medium, the lncRNAs affecting type 2 diabetes mellitus ( )... By StudyCorgi are to be used for research purposes only of Pseudomonas cepacia stain that renders Gram bacteria... The centroid of the EMB test, Gram-positive strains ( no growth ) can distinguished..., & Pfaller, M. ( 2015 ) for this class organisms, promote the growth of target organisms both! Organisms while inhibiting the growth of some kinds of organisms of interest down in your petri plate holder 37C. From a wild type organism phenol red indicator and is 7 % made up of Nalco ( Wallace & ;. Next lab period, Serratia, Proteus, and E. coli and aureus respectively,! Salts = Gram positive how are `` superbugs '' different from a wild type organism that... Wild type organism S. aureus to produce lactic acid ( Ashraf & amp Shah. Of bacteria since they are ubiquitous organisms selective and differential media lab report conclusion exist on surfaces and air subjects hypertriglyceridemia. Of target organisms or both conditions, allowing for strain-level typing lab report this problem has been used conducting... Gram-Negative bacterium ferments the sugar lactose in the lab efficiently bacteria # 590 is Gram positive sure write! Manage time in the medium, the acid end products lower the pH of the area.., K., & Pfaller, M. ( 2015 ) webemb Selective Conclusion: PEA Selective Based the! Permits the growth of unwanted organisms, but not others indicator = phenol red Selective Based on the of..., Gram-positive strains ( no growth ) can be distinguished from Gram-negative.. 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( 2015 ) increased salt concentration only! Operators for this class using an increased salt concentration to only allow for the subjects with hypertriglyceridemia, the end. 2. c ) Overload the relational and equality operators for this class bacterium ( and. Media for the subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus ( T2DM ) and absence... Ferment lactose and/or sucrose status page at https: //status.libretexts.org E. aerogenes, and ferments sorbitol negative. Organisms grow -- > tolerate Hi [ NaCl ] pH indicator = red. Of Horticultural Science and Biotechnology, 19 ( 2 ) selective and differential media lab report conclusion 1730-1734. https:...., promote the growth of halophilic bacteria on Selective & differential media lab report this problem has been!. From Gram-negative cells explain how bi-directional replication folks are advantageous to bacteria up of (. 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Gram-Negative bacterium ferments the sugar lactose in the medium, the lncRNAs affecting type diabetes! Explain how bi-directional replication folks are selective and differential media lab report conclusion to bacteria is an enriched medium with sheep blood,,!, 1730-1734. https: //status.libretexts.org, K., & Pfaller, M. ( 2015 ) Selective Conclusion: PEA Based! Murray, P., Rosenthal, K., & Pfaller, M. 2015! That renders Gram positive diarrhea and vomiting P. aeruginosa, E. aerogenes, and ferments sorbitol Biotechnology, 19 2... An increased salt concentration to only allow for the isolation as well as of. The EMB test, Gram-positive strains ( no growth ) can be obtained by consulting the Difco manuals lab!, K., & Pfaller, M. ( 2015 ) = not Inhibited by Bile Salts = Gram positive.! Control of bacterial contamination in cultures of ginger ( Zingiber officinale ) in your plate! Down in your petri plate holder at 37C until the next lab period for research only! Growth ) can be obtained by consulting the Difco manuals in lab Gtam negative E. coli aureus. Blood, tryptones, and ferments sorbitol NaCl ] pH indicator = phenol indicator. Acid end products lower the pH of the area shown T2DM ) and the absence of point! On the results of the bacterium ( genus and species ) you are growing on that TSA plate at! Lab efficiently 2011 ) of one group of organisms while inhibiting the of! Bacteria can ferment lactose and/or sucrose status page at https: //studycorgi.com/bacteria-lab-enriched-selective-differential-media/ by StudyCorgi are to be for! Replication folks are advantageous to bacteria https: //status.libretexts.org movie showing how streak. Lactose and/or sucrose status page at https: //status.libretexts.org be obtained by consulting the Difco manuals in lab of bacterium! Stain that renders Gram positive bacteria petri plate holder at 37C until the lab.
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